Structured lattice light-sheet microscopy for high-temporal-resolution imaging of axonal action potential propagation

The work will include the development of the optical illumination and detection architecture, the integration of metasurface components for the generation and control of the structured illumination pattern, and the design of the system to ensure compatibility and integration with micro-nano-fluidic devices for sample confinement and controlled positioning. Particular attention will be devoted to the development of strategies for the controlled translation and modulation of the illumination pattern and to the synchronization of different illumination configurations with high-speed image acquisition. In parallel, computational image reconstruction procedures will be developed and optimized using acquisitions obtained with different positions or configurations of the illumination pattern, with the aim of increasing spatial resolution while preserving high temporal resolution

Selection process

Il conferimento dell’incarico avviene sulla base della valutazione delle manifestazioni di interesse pervenute da parte del Responsabile scientifico della ricerca, Dott.ssa Anna Archetti, che valuterà i candidati sulla base dell’attinenza del profilo al progetto • Skills in experimental optics and photonics, with experience in the design, implementation, and operation of optical setups and in the characterization of photonic devices;• Experience in the acquisition and analysis of optical signals, including in biophysical applications, with particular reference to the monitoring of neuronal cells;• Familiarity with microscopy and optical imaging techniques, as well as with micro-nano-fluidic systems integrated into optical configurations.

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